Color Movies with VoxBlast: Calcium Labeled Cardiac Myocyte
(Deconvolved Confocal Image)
This movie shows a 3D reconstruction of a cardiac myocyte in which the calcium has been labeled with a fluorescence dye. The data was captured with a confocal microscope and then deconvolved with MicroTome to highlight the surface structures. VoxBlast was then used to extract the surface from the myocyte. The 3D surface area was calculated as the surface was extracted. Finally, the volume and the ratio of the surface area/volume were calculated. The results showed that
- Estimates of the surface area are incorrect by a considerable amount due to the convolutions in the surface,
- The surface area/volume ratio is off by large amounts due to the estimate, and
- The measurement of the surface area is much more accurate when the data is first deconvolved with MicroTome, even though the data was originally captured with a confocal microscope.
- The movie loop was reduced in size to save download time. The static image shows the detail of the extracted surface, clearly visible in the full size movie.
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