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Color Movies with VoxBlast: Fluorescent Alveolar Septum
Rat Lung Alveoli movies were created using VayTek's VoxBlast software.
The image was acquired with a Biorad laser scanning confocal microscope, using fluorescence. (Biorad MRC 600 at the Integrated Microscopy Resource Center, UW.)
The tissue was processed by freeze fixation--ethanol dehydration--JB4 resin imbedment. Modest image refinement and cleaning was done in NIH image and then the image was imported into VoxBlast, version 1. The opacity algorithm in VoxBlast was used to remove air space.
This research focuses on capillary-level structures of the lung. This computer reconstruction shows not just the surface features, but also the all of the capillaries, including the backside of the volume, in true 3D rotation.
Click here to download a larger version of this movie loop as a self-extracting, zipped AVI file. (12.8 MB)
Click here to download a QuickTime movie loop, with the lung tissue transparent. (2.6 MB)
Click here to download a larger version of this movie loop as a QuickTime file, compressed with MacBinary II. (15.7 MB)
The VoxBlast movie player is required to view .EXE VoxBlast movies. If you do not already have the player, you can download it here.
Click here to download the Windows version of the VoxBlast movie player (75,000 bytes).
If you would like a Macintosh or UNIX version of the movie player, please contact VayTek.
Tel 641-472-2227 Fax 641-472-8131 Email vaytek@vaytek.com
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