|
|
Color Movies with VoxBlast: Oogenesis in the African Clawed Frog, Xenopus laevis
Data courtesy of David L. Gard, Ph.D., Dept. of Biology, University of Utah, Salt Lake City, Utah
The sample above was rendered from data collected from a confocal microscope. The movie depicts a stage of oogenesis in the African clawed frog, Xenopus laevis.
The following paragraph is an excerpt from Dr. Gard's Website:
The 3-D reconstructions seen here as video animations were generated using VoxBlast software from VayTek, Inc., running under Windows for Workgroups 3.11 on a 90MHZ Pentium personal computer equipped with 64 MB of physical RAM. Rendering times varied from 10-60 seconds for smaller data sets (less than about 40 MB) that can be loaded completely into RAM, to 15 minutes for 150-175 MB data sets that require the use of the windows swap file (the size of the data set includes the "real" optical sections, plus "interpolated" sections included to give the correct XYZ aspect ratio...a data set including 10 full-screen "real" images, with four "interpolated" images between each is about 20 MB total size). The animated video clips were generated frame-by-frame in VoxBlast by sequentially reconstructing views differing one degree in azimuth, and were then converted to QT and AVI formats in Adobe premiere.
Click here to download a larger version of this QuickTime movie loop (10,614,000 bytes)
Data courtesy of David L. Gard, Ph.D., Dept. of Biology, University of Utah, Salt Lake City, Utah
The sample above was rendered from data collected from a confocal microscope. The movie depicts a stage of oogenesis in the African clawed frog, Xenopus laevis. The female egg shows a complex array of microtubules, which form tracks for transporting molecules throughout everything inside a cell except its nucleus. More than 50 contiguous sections were acquired and then reconstructed with VayTek's VoxBlast to create a three-dimensional volume. The research focuses on understanding microtubule regulation in oocytes. A combination of advanced imaging techniques allows the researcher to see complex spatial and temporal patterns of how the microtubules assemble and organzie themselves.
The VoxBlast movie player is required to view .EXE VoxBlast movies. If you do not already have the player, you can download it here.
Click here to download the Windows version of the VoxBlast movie player (75,000 bytes).
If you would like a Macintosh or UNIX version of the movie player, please contact VayTek.
Tel 641-472-2227 Fax 641-472-8131 Email vaytek@vaytek.com
© 2000 VayTek Inc. Information may be reproduced only with permission from VayTek Inc.