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VayTek offers Advanced Image Processing Systems, including both hardware and software for:

• Microscopy
• Industrial Inspection
• Medical Imaging
• Quality Control
• Non-Destructive Testing
• Deconvolution of Confocal Images
• 3D Volume Visualization and Measurement

VayTek, Inc.
305 West Lowe Ave.
Fairfield, IA
Tel 641-472-2227
Fax 641-472-8131
Email vaytek@vaytek.com

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Application Note

 Deconvolution and 3-D Reconstruction:
with an empirically sampled PSF

This image is from a Hippocampal Organotypic Culture (HOTC) that was processed for Neurofilament (silver intensified) via immunohistochemistry. The neuron was sampled at 0.2 micron intervals, with a total of 36 planes. The volume was deconvolved using the Constrained Iterative algorithm in VayTek's MicroTome software, with an empirically sampled PSF (point spread function) from the objective used (63x, oil Leica Laborlux). 3D reconstruction was accomplished with VayTek's VoxBlast software.

Work in the Kraig laboratory at the University of Chicago Department of Neurology centers on understanding glia, fundamental cells within the brain. Laboratory personnel concentrate their efforts on how astrocytes and microglia orchestrate the response of neural tissue to physiological and pathological stimuli. Questions actively pursued in the laboratory include: what role astrocytes and microglia have in making, maintaining, and modifying synapses; how these cells influence the evolution of ischemic brain injury; and how they regulate the capacity for regeneration after ischemic injury. In addition, lab personnel study how glia may participate in the development of inflammation from Alzheimer's disease.

The Kraig lab includes its director, Richard P. Kraig, Ph.D., M.D. (Professor of Neurology, Pharmacological & Physiological Sciences & the Committee on Neurobiology, Co-chair for the Department of Neurology); Phillip E. Kunkler, Ph.D. (Research Associate, Instructor); Mr. Raymond Hulse (Analyst); and Mrs. Marcia P. Kraig (Senior Research Technician).

Dr. Phillip Kunkler performed maintenance and immunohistochemistry for this sample. Additional image processing by Mr. Raymond E. Hulse.