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Deconvolution Software
Haze Removal Software from VayTek


Before Deconvolution

After Deconvolution

These color images consist of three separate 12 bit grayscale images, each captured at a different wavelength for three different dyes: FITC for the actin fibers, rhodamine for a protein, and DAPE for the nucleus. Images were captured at 6 different vertical depths at 1 micron steps. Each color channel was processed separately, and a topview composite image was created. By compositing several images from different vertical depths in the image a more complete view of the specimen is produced. The composite view of the images with the haze removed is much more revealing than a topview of the unprocessed images.


Raw Image

Deconvolved Image
In many cases, state-of-the-art deconvolution algorithms can equal or augment the capabilities of a scanning confocal microscope. This image of a computer chip was deconvolved using VayTek's MicroTome software. The word "deconvolve" means to untangle or unwind. A deconvolution algorithm is a systematic procedure for removing noise or haze from an image. Digital Deconvolution, uses software to remove out-of-focus haze from microscope images. For many applications, the results are comparable to those produced by much more expensive scanning confocal microscopes. The digital deconvolution approach offers more flexibility and a wider range of applications.

Digital Deconvolution Microscopy consists of four separate steps:
  • Data acquisition (see Data Acquisition Issues)
  • Haze removal or deconvolution
  • 2D image analysis
  • 3D reconstruction (see VoxBlast)
  • For more, fundamental information see our FAQ
VayTek provides a variety of software and hardware packages that address the issues for each step of the process.

MicroTome for Windows and MicroTome Power Mac, address each step separately and give the user the most power and the most flexibility in data acquisition, deconvolution and post-processing.

Proteus is the high-end, high-tolerance system incorporating the most accurate cameras, algorithms and electronics to produce highly detailed images. Because Proteus uses a cooled CCD camera it can capture images that are dimmer than those typically seen on a scanning confocal microscope.

Customers who purchase MicroTome often need additional programs and/or hardware for data acquisition, (see VolumeScan) and post-processing. VayTek also offers data acquisition packages and turnkey systems that include a computer, camera, frame-grabber board, stage stepper motor, and all the software necessary to control the hardware, deconvolve the images, and then process them.

Each of these products is described in detail. VayTek personnel are available for consultation on system integration and will work with you to retrofit your current system with VayTek's digital imaging technology

Software for Optical Microscopy

  • FAQ What is Deconvolution?
  • MicroTome Specifications:
    • Windows
    • Power Mac & Macintosh
  • Technical Note: Deconvolution Algorithms
  • Recommended Step Size for Deconvolution
  • Application Notes
  • Selected Papers
  • Data Acquisition Issues
  • Image Acquisition Software & Peripherals Control


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