Compare Camera Images

• 40x .75NA lens
• Olympus BX60 microscope
• Mercury lamp
• 500 ms exposure time for all images
• A new part of the specimen was used for each capture to avoid bleaching out the specimen
• The specimen was a triple stained slide prepared by Molecular Probes. It was a commercially purchased stain: Bovine pulmonary artery enothelial cells, fitc, dapi, texas red; green is tubulin, red is F-actin, and blue are nuclei.
• Some researchers don't have programs to read 12 bit images, so each captured image was converted to 8 bits. The 8 bit image lets the viewer see the image, but truncates the dynamic range.
• The comparison images are quite large, so download time may be significant. Cooke Sensicam, 8 bit images Cooke PixelFLY, images from manufacturer Optronics Magnafire sample image (not acquired using the standardized capture conditions listed above, but an image from the Optronics promotional archives). Roper Sensys, 8 bit images Roper Quantix, 8 bit images Roper Micromax, 8 bit images Hamamatsu, 8 bit images