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8 Bit Images Acquired with a Hamamatsu Camera

Using standardized capture conditions, VayTek has produced a set of comparison images for some of the most popular cameras for digital imaging systems for science, medicine, research and microscopy. The capture conditions were:

• 40x .75NA lens
• Olympus BX60 microscope
• Mercury lamp
• 500 ms exposure time for all images
• A new part of the specimen was used for each capture to avoid bleaching out the specimen
• The specimen was a triple stained slide prepared by Molecular Probes. It was a commercially purchased stain: Bovine pulmonary artery enothelial cells, fitc, dapi, texas red; green is tubulin, red is F-actin, and blue are nuclei.
• Some researchers don't have programs to read 12 bit images, so each captured image was converted to 8 bits. The 8 bit image lets the viewer see the image, but truncates the dynamic range.

8 bit Dapi

8 bit FITC

8 bit Rhodamine