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Cameras Definitions
Cameras Deconvolution
Application Notes
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VayTek, Inc.
505 North 3rd St.
Suite 200
Fairfield, IA 52556
Tel 641-472-2227
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Compare Cameras
Intro
Compare Cameras 1
Compare Cameras 2
Compare Cameras 3
Compare Cameras 4
Compare Cameras 5
Compare Cameras 6
Compare Cameras 7
Compare Cameras 8
Cameras Definitions
Cameras Deconvolution
Compare Cameras - View Images
Compare Images Acquired with Different Cameras
Using standardized capture conditions, VayTek has produced a set of comparison images for some of the most popular cameras for digital imaging systems for science, medicine, research and microscopy. The capture conditions were:
- 40x .75NA lens
- Olympus BX60 microscope
- Mercury lamp
- 500 ms exposure time for all images
- A new part of the specimen was used for each capture to avoid bleaching out the specimen
- The specimen was a triple stained slide prepared by Molecular Probes. It was a commercially purchased stain: Bovine pulmonary artery enothelial cells, fitc, dapi, texas red; green is tubulin, red is F-actin, and blue are nuclei.
- Some researchers don't have programs to read 12 bit images, so each captured image was converted to 8 bits. The 8 bit image lets the viewer see the image, but truncates the dynamic range.
The comparison images are quite large, so download time may be significant.
Cooke PixelFLY, images from manufacturer
Optronics Magnafire sample image (not acquired using the standardized capture conditions listed above, but an image from the Optronics promotional archives).
Articles recommended by VayTek that are available on this website:
- Appropriate Light Sources: Advantages of digital haze removal
- Single Image Haze Removal from an Archived File
- Haze Removal from Color Images and Composite Top Views
- A Comparison of Digital Deconvolution and Scanning Confocal Images
- Data Acquisition Issues and Cameras Appropriate for Deconvolution
- Data Acquisition Issues: Image acquisition techniques
- Introduction to Digital Systems for Microscopy: Microscopy fundamentals
- Selected Papers: Limits of depth measurements on stereo pairs
- Technical Note: Deconvolution algorithms and how they work
- What cameras are suitable for deconvolution?
- What resolution should I have for my camera?
