VayTek, books, microscopy, confocal microscopy, medical imaging, video microscopy, fluorescence microscopy

The books listed below are VayTek's recommendations for Microscopy and Confocal Microscopy.
Links below lead to amazon.com website for purchasing. See book links in the lefthand column for additional recommendations.

Video Microscopy : The Fundamentals
by Shinya Inoue, Kenneth R. Spring

Hardcover - (August 1997) 764 pages

Book Description
This classic has been throughly revised and expanded to reflect technological advances, but it still emphasizes the fundamentals, with extensive reviews of optical microscopy, video detectors, and display devices. Three completely new chapters deal with solid-state detectors, both video-rate and slow scan, image intensifiction; and the color video signal and color video cameras. Another new chapter treating the practical aspcts of the design and use of video microscope systems is especially notable. This edition fetures over 500 illustrations (including 126 half-tones), a glossary of items, detailed tables of specifications, and a list of

Fluorescence Microscopy (Microscopy Handbooks, 40)
by H. J. Tanke, Brian Herman

Paperback - 144 pages 2nd edition (May 1998)
Springer Verlag; ISBN: 0387915516 ; Dimensions (in inches): 0.43 x 9.20 x 6.20

Book Description : Scientists use fluorescence microscopy to biochemically label and then detect molecules within the cell as part of basic biomedical research and for diagnostic purposes. Fluorescence Microscopy is the revised, updated and expanded second edition of a best-selling introductory text on the use of fluorescence microscopy in cell biology. The book covers the fundamental principles of fluorescence and their application to fluorescence microscopy. A detailed description of the fluorescence microscope is given too. Application to immunofluorescence, in situ hybridization, and photomicrography are presented in full, practical details. Specific topics covered include: Fundamentals of fluorescence; Components of fluorescence microscopes; Quantitative fluorescence microscopy; Green fluorescent proteins; Fluorescence in situ hybridization (FISH); Digitized video microscopy; Single and multiphoton microscopy; Fluorescence photomicroscopy; Fluorescence Microscopy.

Confocal Scanning Optical Microscopy and Related Imaging Systems
by Timothy R. Corle (Editor), Gordon S. Kino (Editor)

Hardcover - 352 pages (September 1996)
Academic Pr; ISBN: 0124087507 ; Dimensions (in inches): 0.80 x 9.22 x 6.13

from Book News, Inc. : Introduces scientists and engineers to the field of scanning optical miscroscopy in order to overcome the problems associated with submicrometer imaging of complex 3D structures. Concentrates mainly on the confocal scanning optical microscope (CSOM), and the optical interference microscope (OIM), although it does discuss the theory and design of the near-field scanning optical microscope (NSOM). Covers practical aspects of building a CSOM or OIM, and considers the applications of these instruments to phase and biological imaging, semiconductor inspection, and metrology. Also contains a theoretical discussion of the depth and transverse resolution, emphasizing practical results of the theoretical calculations, and their uses in understanding the operation of the microscopes. -- Copyright © 1999 Book News, Inc., Portland, OR All rights reserved

Fluorescence Microscopy
by F. W. D. Rost

This is the second volume of a two-volume work on fluorescence microscopy that covers all aspects of the subject including instrumentation, applications, and history. Volume 2 deals with applications of fluorescence microscopy in many fields. It includes chapters on fluorocarbons and autofluorescence. An invaluable appendix contains an alphabetical list of fluorochromes and gives information on chemical structure, fluorescence properties, applications, and suitable filter combinations.

Confocal Laser Scanning Microscopy by C. J. R. Sheppard, et al
Paperback - October 1997

Book Description: Describes the principles of the technique and illustrates applications in physical and biomedical sciences. Covers Image formation in confocal microscopy, Performance of the confocal microscope, Biological and industrial applications. Paper. DLC: Confocal microscopy.

Reviewer: A reader from Storrs, CT United States
An excellent primer for those who have just a little knowledge, but are missing the background to handle CLSM data with confidence. Readers with some knowledge of the jargon of microscopy will find the book to be an easier read, but this reviewer had no prior knowledge and found the book extremely helpful. It gives enough fundamental information to let the potential CLSM user know where s/he is going to get into trouble. If you are going to image something for the first time, get this book. It'll keep you from interpreting artifacts.

Confocal and Two-Photon Microscopy : Foundations, Applications, and Advances by Alberto Diaspro (Editor)

Hardcover - November 2001

from Book News, Inc. : This collection of 26 papers treats both the basics as well as new developments in governing resolution, contrast, and 3D image formation and processing. It offers current information about fluorescent molecules, imaging software, and new commercial trends. The applications are in both the medical research and semiconductor fields. Some of the topics are the role of pinhole size in high- aperture two- and three-photon microscopy, cellular perturbations during multiphoton microscopy, stereological methods for estimating geometrical parameters of microscopic structure by 3D imaging, confocal imaging of neuronal growth and morphology in brain slices, super-resolution in DVD optical storage, and photobleaching by confocal microscopy.Book News, Inc.®, Portland, OR

Handbook of Biological Confocal Microscopy (The Language of Science) by James B. Pawley (Editor)

Hardcover - February 1995

from Book News, Inc.: New edition of a reference originally published in 1989 and revised in 1990. The present edition includes 37 chapters (15 updated from the first edition, 21 new ones, and an annotated bibliography), and is nearly three times as long as the original. In particular, it reflects an increased recognition of the extent to which the resolution and signal strength of confocal images can be degraded by spherical aberration introduced whenever there is a refractive-index mismatch, such as that occurring when an oil-immersion objective is used with an aqueous specimen. There is a tutorial chapter for novice users, and there are two appendices, the first of which describes the relationship between real-space and optical coordinates, while the second provides a compilation of the optical path layouts of the major commercial confocal instruments. Annotation copyright Book News, Inc. Portland, Or.

Reviewer: rda9046@utarlg.uta.edu from Arlington, TX
This is a great reference book. A great place to start if you want to learn about confocal microscopes. The chapters are written by experts in their specific field of confocal microscopy. The book is also well referenced so that you can easliy continue your search for knowledge on confocal microscopy. I also found the appendix interesting, they list most of the existing confocal systems and there specs. This is useful if you plan on purchasing one. This book is also useful if you plan on building your own.

Confocal Microscopy Methods and Protocols by Stephen W. Paddock (Editor)

A laboratory-bench reference that walks biomedical researchers and students familiar with visible-light microscopy and the basic use of computers through the stages of imaging techniques that virtually eliminates the out-of-focus fluorescence that makes conventional techniques difficult or suspect. Includes information on sampling and staining samples and on manipulating, presenting, and publishing the realized image. Rather than detail technical aspects, which are described elsewhere and change as new techniques and equipment are brought on-line, focuses on a selection of protocols for the most common plant and animal models used in research. among these are worms, sea urchins, flies, yeast, frogs, and zebrafish. Except four color plates,the illustrations are in black and white. The references include Web sites. Book news, Inc. copyright, Portland, OR

Multidimensional Microscopy by P.C. Cheng, et al
Out of Print--Limited Availability
Hardcover (January 1994)
Springer Verlag; ISBN: 0387941185

Book Info : State University of New York, Buffalo. Current research in microscopic technology, with biomedical applications. Includes confocal, x-ray, and low voltage scanning electron microscopy, as well as image restoration and reconstruction. 37 contributors, 17 U.S. contributors. Illustrated.

Proceedings of three-dimensional microscopy : image acquisition and processing IV : 12-13 February 1997, San Jose, California Out of Print--Limited Availability - 274 pages SPIE; ISBN: 0819423955
Book Info : Describes the principles of the technique and illustrates applications in physical and biomedical sciences. Covers Image formation in confocal microscopy, Performance of the confocal microscope, Biological and industrial applications

Cell Biological Applications of Confocal Microscopy by Brian Matsumoto (Editor), Paul T. Matsudaira (Editor), Leslie Wilson (Editor)
Spiral-bound - 375 pages 1st edition Vol 038 (January 15, 1993)
Academic Pr; ISBN: 0124804306

Book Info
Instructs researchers on methods of preparing biological material for confocal microscopy. Provides specific examples of applications of confocal microscopy to cell biological problems.

Confocal Microscopy (Methods in Cell Biology, Vol 69) by Brian Matsumoto
Paperback 2nd Rev edition Vol 69 (October 2002)
Academic Press; ISBN: 0125441681

Protein Localization by Fluorescence Microscopy: A Practical Approach by Victoria J. Allan (Editor)
Paperback: 231 pages
Publisher: Oxford University Press; ISBN: 0199637407; 1st edition (February 15, 2000)

Book Description: There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques-- including live cell work--against the potential pitfalls, offering invaluable "tricks of the trade" along the way.

Book Info: Univ. of Manchester, UK. Provides detailed advice on all aspects of the subject, from choosing the right equipment, to interpreting results. For microscopists. Softcover, hardcover also available.

Focus on Multidimensional Microscopy by P.C. Cheng, Wu. W.L., T.H. Lin, J.L. Wu (Editor)
Out of print. Limited availability
Hardcover (January 1994)
Springer Verlag; ISBN: 0387941185
Book Info : State University of New York, Buffalo. Current research in microscopic technology, with biomedical applications. Includes confocal, x-ray, and low voltage scanning electron microscopy, as well as image restoration and reconstruction. 37 contributors, 17 U.S. contributors. Illustrated.

Imaging Living Cells (Springer Lab Manual) by Rosario Rizzuto (Editor), Cristina Fasolato (Editor)
Hardcover: Spiral edition (March 1999)
Publisher: Springer Verlag; ISBN: 3540650512

Book Description: Improved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together with classical probes. Novel labelling strategies, better software for image enhancement and analysis are now available and allow image acquisition with greater speed and precision. This lab manual, intended as for bench-top use, is suitable for both scientists and graduate students, combines an update on the most advanced imaging procedures with detailed protocols. Examples, cleverly selected from the wide repertoire of cell pyhsiology, cover different functional aspects such as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.

Selected Papers on Confocal Microscopy (Spie Milestone Series, Vol MS 131) by Barry R. Masters (Editor)
Hardcover: (June 1996)
Publisher: Society of Photo-optical Instrumentation Engineers; ISBN: 0819423726

Confocal Microscopy (Methods in Enzymology, Volume 307) by P. Michael Conn (Editor)
Hardcover: 696 pages
Publisher: Academic Press; ISBN: 0121822087; (August 1999)

Book Description: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.

From the Publisher: Praise for the Series "The Methods in Enzymology series represents the gold-standard." (Neuroscience) ; "Incomparably useful." (Analytical Biochemistry) ; "...including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." (Bio/Technology) ; "...It should be on the shelves of all libraries in the world as a whole collection." (Chemistry in Industry); "The appearance of another volume in that excellent series.

Visualization in Biomedical Microscopies: 3-D Imaging and Computer Applications by Andres Kriete (Editor)
Paperback: September 1992
Hardcover: August 1992
Publisher: Vch Pub; ISBN: 1560812222

From Book News, Inc.: The contributors discuss the state-of-the-art in computer-aided 3D imaging design and applications in three areas: acquisition and visualization of serial section images; visualization and analysis in confocal microscopy; and multi-dimensional image generation and representation in advanced microscopy.

Principles of Three-Dimensional Imaging in Confocal Microscopes by Min Gu
Hardcover: December 1996
Publisher: World Scientific Pub Co; ISBN: 9810225504

Book Description: This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functions.

Three-Dimensional Confocal Microscopy : Volume Investigation of Biological Specimens (Cell Biology) by John K. Stevens (Editor), Linda R. Mills (Editor), Judy E. Trogadis (Editor)
Hardcover: 507 pages
Publisher: Academic Press; ISBN: 0126683301; (August 1994)

From Book News, Inc.:The capacity of confocal microscopy for obtaining high-resolution images of very thin sections allows researchers to look inside the cell at subcellular structures, and even dynamic events, previously invisible using conventional light microscopy. This book familiarizes readers with its potential for quantitative analysis of the 3- dimensional architecture of tissues, cells, organelles, and macro- molecular structures, a field newly defined as volume investigation. The applicability of these new technologies to a wide range of biological and clinical problems is also demonstrated. Annotation copyright Book News, Inc. Portland, Or.

Book Description: University of Toronto, Ontario, Canada. Reference for cell biologists or other laboratory investigators on the application of new technologies in microscopy to a wide range of biological and clinical problems. 49 contributors, 27 U.S.

Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence by Peter H. Bach, C. Hugh Reynolds, Jessica M. Clark
Hardcover: July 1993
Publisher: Plenum Pub Corp; ISBN: 0306444976

From Book News, Inc.: In 25 papers from a European workshop in London, England, April 1992, cell biologists explore how the microinjection of defined material can be used to manipulate the fundamental biochemistry of specific living cells. The techniques described tend to be highly sophisticated and highly expensive, so would interest researchers with access to advanced laboratories; they include the characterization of the Zea mays embryo sac using fluorescent probes and the microinjection of Lucifer Yellow, and the quantitative localization of HIV proteins in mammalian cells. Annotation copyright Book News, Inc. Portland, Or.

Electronic Light Microscopy: The Principles and Practice of Video-Enhanced Contrast, Digital Intensified Fluorescence, and Confocal Scanning Light by David Shotton (Editor)
Hardcover: 368 pages
Publisher: John Wiley & Sons; ISBN: 0471560774; February 1993

From Book News, Inc.: A practical laboratory reference text that offers guidelines for applying digital image processing methods to the analysis of electronic light microscope images of fixed and living biological specimens. Topics include image acquisition and sampling, image averaging and background subtraction, object tracking, ratio imaging, computational deblurring of both conventional and confocal images, three-dimensional image processing, and animation. Many black and white illustrations and nine pages of color plates are included. Annotation copyright Book News, Inc. Portland, Or.

The publisher:Uses a methods-oriented approach to examine the principles and practice of confocal and video microscopy, including computer reconstruction in three-dimensional fluorescence microscopy.

Digital Image Analysis of Microbes: Imaging, Morphometry, Fluorometry and Motility Techniques and Applications by M.H.F. Wilkinson (Editor), F. Schut (Editor)

Hardcover: 578 pages
Publisher: John Wiley & Son Ltd; ISBN: 0471974404; (May 15, 1998)

The publisher: Providing specific knowledge in the theory of image analysis, optics, fluorescence, and imaging devices in biomedical laboratories, this timely and indispensable volume focuses on the theory and applications of detection, morphometry, and motility measurement techniques applied to bacteria, fungi, yeasts and protozoa.

Image Analysis in Histology: Conventional and Confocal Microscopy by R. Wootton (Editor), David Springall (Editor), Julia Polak (Editor)
Hardcover: 425 pages 1st edition (January 15, 1995)
Publisher: Cambridge University Press; ISBN: 0521434823

Book Description: This volume provides a timely and useful introduction to the theory and practical application of image analysis in histology. This powerful research technique can be used to detect not only stored products in a cell (immunocytochemistry) but the synthetic machinery and the genes that control it (in situ hybridisation), as well as the specific binding sites that act as receptors for a molecule following its release (in vitro autoradiography). The book provides a good introduction for beginners before looking in greater detail at more advanced material in selected areas.The volume highlights the importance of technique in gathering quantitative information. The book is divided into four sections: introductory material, image acquisition, image processing, and applications. the applications areas include quantitative immunochemistry, quantification of nerves and neurotransmitters and automated grain counting in in situ hybridisation histochemistry.

Book Info: Royal Postgraduate Medical School, London, U.K. Introduction to a powerful research technique for investigators in histology and pathology. Based on a course at the Royal Postgraduate Medical School in London.

Three-Dimensional Microscopy: Image Acquisition and Processing II : 9-10 February 1995 San Jose, California (Proceedings of Spie--The International s by Tony Wilson (Editor), Carol J. Cogswell (Editor)

Paperback: June 1995
Publisher: Society of Photo-optical Instrumentation Engineers; ISBN: 0819417599

Confocal Microscopy (not available on amazon.com) by T. Wilson (Editor) (Hardcover - October 1997)
Articles recommended by VayTek that are available on this website:

Appropriate Light Sources: Advantages of digital haze removal
Single Image Haze Removal from an Archived File
Haze Removal from Color Images and Composite Top Views
A Comparison of Digital Deconvolution and Scanning Confocal Images
Data Acquisition Issues and Cameras Appropriate for Deconvolution
Data Acquisition Issues: Image acquisition techniques
Introduction to Digital Systems for Microscopy: Microscopy fundamentals
Selected Papers: Limits of depth measurements on stereo pairs
Technical Note: Deconvolution algorithms and how they work
What cameras are suitable for deconvolution?
What resolution should I have for my camera?