Before Deconvolution


After Deconvolution

More information is available in color images than in grayscale images. Thus the ability to remove the haze from color images is an advantage.

With VayTek's haze removal algorithms it is possible to remove the haze from both 8 bit grayscale and 24 bit RGB color images. The color images can be a single composite color image, or three separate (red, green and blue) component images. If separate color component images are used, the images can be 3 separate 12 bit images.

Color image processing is automatic in VayTek's MicroTome software.

The color image at the left consists of three separate 12 bit grayscale images, each captured at a different wavelength for three different dyes: FITC for the actin fibers, rhodamine for a protein, and DAPE for the nucleus. Images were captured at 6 different vertical depths at 1 micron steps.

Each color channel was processed separately, and a topview composite image was created. By compositing several images from different vertical depths in the image a more complete view of the specimen is produced. The composite view of the images with the haze removed is much more revealing than a topview of the unprocessed images.