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VayTek offers Advanced Image Processing Systems, including both hardware and software for:

• Microscopy
• Industrial Inspection
• Medical Imaging
• Quality Control
• Non-Destructive Testing
• Deconvolution of Confocal Images
• 3D Volume Visualization and Measurement

VayTek, Inc.
505 North Third Street
Suite 200
Fairfield, IA 52556
Tel 641-472-2227
Fax 641-472-8131
Email vaytek@vaytek.com

Copyright All information on this World Wide Web site is copyrighted and may be reproduced only with permission from VayTek, Inc.

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The eight panel figure below is made up of images generated in the same living cell of heart tissue. A digital imaging system from VayTek was used to diagnose single cell responses to various drugs (a physiologic response) and also analyze changes in intracellular structure in the same cell.

The top panels represent light images of two vascular smooth muscle cells. The left cell is a healthy cell and the right cell is from a diseased artery. The difference in morphology is clear.

The second row of images is a 3-D reconstruction of the same cell loaded with an internal membrane marker and a marker for DNA. The cell has been rotated and split in half so that one can see into the live cell. The differences in organelle distribution between cells is clear.

The third row is an X-Z, 3-D depiction of the same cell, showing that in healthy cells, internal organelles invaginate the nucleus and this does not occur in diseased cells.

Tthe fourth panel is a physiological response of these same live cells to drug X. Here the calcium responses are measured using a fluorescent calcium probe. Clearly, the diseased cells respond to drug X and the healthy cell does not.

The focus of the research is to diagnose diseased cells in the arteries of heart attack patients. Viewing living (not fixed) cells and looking at changes in morphology, organelle distribution, DNA content and physiological responses represents a great achievement.

 

Courtesy of Brain R. Wamhoff, Dept. of Physiology, Dalton Cardiovascular Research Center, Columbia, MO, USA. Image acquisition and processing by VayTek's software, including MicroTome and VoxBlast.

Please note that the panel of figures shown above was reduced in both size and resolution for website presentation. Researchers interested in viewing the panel at 300 dpi, and in full size, should contact Brian Wamhoff at the Dalton Cardiovascular Research Center.
At the time of this website page construction, publication of similar images, along with a technical paper was soon to be accomplished. Inquire of Mr. Wamhoff for details.