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VayTek offers Advanced Image Processing Systems, including both hardware and software for:

• Microscopy
• Industrial Inspection
• Medical Imaging
• Quality Control
• Non-Destructive Testing
• Deconvolution of Confocal Images
• 3D Volume Visualization and Measurement

VayTek, Inc.
505 North Third Street
Suite 200
Fairfield, IA 52556
Tel 641-472-2227
Fax 641-472-8131
Email vaytek@vaytek.com

Application Note

 VoxBlast(TM) 3-D Reconstruction: Examples and Tips

Mycelium Fungus, a common antigen in everyday allergies. 3-D reconstruction by VoxBlast   24-hour preserved lung tissue. Green indicates live signal and red indicates dead. Rendered in Voxblast Images courtesy of Lance Rodenkirch. All images in this application note were processed with a Macintosh computer.

"VayTek's VoxBlast is easy to use and has an opacity setting that lets me see inside the data set. Without this feature some structures would not be visible." -- Lance Rodenkirch, Lab Manager, W.M. Keck Neural Imaging Lab The top image that you see on the left is a confocal Z-series of Mycelium Fungus, a common antigen in everyday allergies. The data was acquired to look at structural changes. It is roughly 35 layers at 0.5 um increments at 60x. The image was collected on a Biorad 1024 confocal at 512x512. This image looks both interesting and impressive in 3-D since the fluorochrome and the background have a huge difference in intensity. Tip: a large difference in intensity usually results in a superior 3D image. The bottom image that you see on the left is lung tissue. The researcher applied a live/dead kit (Molecular Probes, Eugene, OR) designed for isolated cells to the organ level. The green component indicates live signal (from an esterase enzyme) and the red indicates a dead signal (compromised cell membrane, exposed nucleic acids). The image is from a 24 hour preserved lung. Two fluorochromes were used, then the image was rendered in VayTek's VoxBlast software. The primary advantage seen in this method of data acquisition and image processing is that the researcher can use the confocal to view "into" the preserved tissue; and with VoxBlast's opacity capabilities, remove voxels that were hiding information as to what cells were being injured first under preservation periods. This cellular death is what releases cytokines that may play a role in organ rejection. This work was done for the Univeristy of Wisconsin Organ Preservation Team. The image was collected on a Biorad 1024-60x; 512x512; 0.5 um Z increments.