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This movie was created with VayTek's VoxBlast 2.1 on a Macintosh
PPC7300/200.
TLive cultured tobacco cells stained with the vital fluorescent membrane dye, FM-143. They were viewed under a Bio-Rad MRC-1024 confocal microscope using an Olympus 40X, NA1.0 water immersion objective. The reconstruction is from 77 images representing approximately 23 microns depth. VayTek's 3D volume visualization and reconstruction software, VoxBlast, was used to create the movie. VoxBlast is available for Windows, Macintosh, and UNIX platforms. For website presentation, the original movie was flattened with FlattenMoov, reduced by 60 percent, and several cycles of rotation were trimmed off each end. In addition, the lower resolution of 8 bits was chosen to speed download time. To download the full-sized and complete movie, click here for a QuickTime version that is 12.1 MB. Click here to download a shorter version for Macintosh only, 7.6 MB. Click here to download a 5.8 MB movie that shows different settings used to display the cell. The 5.8 MB movie also has a more complex rotation than the other two movies. It is in ".mov" format and will play on either a Windows or Macintosh platform. Image processing assisted by Glen MacDonald
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