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Who We Are
VayTek offers Advanced Image Processing Systems, including both hardware and software for:
- Microscopy
- Industrial Inspection
- Medical Imaging
- Quality Control
- Non-Destructive Testing
- Deconvolution of Confocal Images
- 3D Volume Visualization and Measurement
VayTek, Inc.
505 North Third Street
Suite 200
Fairfield, IA 52556
Tel 641-472-2227
Fax 641-472-8131
Email vaytek@vaytek.com
Application Note
Light Sources
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One of the advantages of digital haze removal is that images can be captured using any appropriate light source. There are several implications of this point:
- It is not necessary to use lasers. Conventional light sources
can be used. This can result in less photobleaching.
- Wavelengths not available with lasers can be used. For example,
ultra violet sensitive dyes, such as fura, are appropriate.
- Using filters it is possible to tune excitation wavelengths
precisely to the dyes being used.
- A laser source can be used, if desired. A pulsed laser can be used in some instances which practically eliminates photobleaching.
The example image shows a leech embryo cell that was micro-injected with a rhodamine dextran conjugate dye which photobleached quickly. The parent cell, in the upper right, divided and produced the daughter cell in the lower left. The daughter cell had a smaller amount of the dye and was even more difficult to image.
When a laser scanning confocal was used, the specimen bleached after one scan. Using a conventional light source, a shutter and the proper filters, eleven images at different vertical depths were acquired before the dye quenched. The digitally processed image revealed more detail than the single laser scanned image.
